Abstract
Objective
The purpose of this study was to explore the feasibility of using ultrasound-targeted
microbubble destruction to treat liver fibrosis induced by hepatocyte growth factor
(HGF).
Methods
Forty Wistar rats were divided into five groups after the models of liver fibrosis
were prepared: (1) HGF, ultrasound, and microbubbles (HGF+US/MB); (2) HGF and ultrasound
(HGF+US); (3) HGF and microbubbles (HGF+MB); (4) HGF (HGF); and (5) model alone (MA).
All rats were killed after being transfected for 14 days. Recovery of the liver was
detect by diffusion-weighted imaging (DWI) and pathological methods. Collagen I expression
was detected by immunohistochemistry. Hepatocyte growth factor expression in the liver
was detect by western blotting.
Results
The results of DWI and pathological examination showed the recovery of liver in HGF+US/MB
group were better than those of other groups. In HGF+US/MB group, collagen I expression
was less, and HGF protein was the highest among all the groups.
Conclusions
Ultrasound-targeted microbubble destruction could deliver HGF into the fibrotic liver
and produce an antifibrosis effect, which could provide a novel strategy for gene
therapy of liver fibrosis.
Keywords
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Article info
Publication history
Published online: August 26, 2009
Accepted:
April 25,
2009
Received:
April 5,
2009
Footnotes
☆This study supported by The program of National Natural Sciences Foundation of China (No. 30770566) and (No. 30770565).
Identification
Copyright
© 2009 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
